Asn-Asn-Trp-Asn-Asn/960129-66-2/GT Peptide/Peptide Mea hoʻolako

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ʻO ka make cell programmed i ka bacteria e hilinaʻi ana i kahi ʻōnaehana kamaʻilio intercellular i kapa ʻia ʻo quorum sensing.ʻO nā mea make ʻē aʻe (EDFs) he mau pentapeptides laina i hana ʻia a hoʻokuʻu ʻia e ke kamaʻilio ʻana i nā cell e, i ka wā e lawa ai ka ʻike, hoʻāla i nā ala make cell i loko o kahi ʻāpana o nā cell.Hoʻopili ia i ka pH kiʻekiʻe, ka wela kiʻekiʻe, a me nā kūlana koʻikoʻi ʻē aʻe.Ma kahi o 2.5 ng/ml, ua hōʻike ʻia ʻo EDF e hoʻolaha i ka make ʻana o ka maʻi mīkiaf-mediated cell, e hōʻemi nui ana i ka nui o ka heluna kanaka ma nā moʻomeheu Escherichia coli.

Nā kikoʻī

ʻAno: Keʻokeʻo a keʻokeʻo pauka

Maʻemaʻe (HPLC):≥98.0%

Hoʻokahi haumia:≤2.0%

Maʻiʻo Acetate (HPLC): 5.0%～12.0%

Waiwai (Karl Fischer):≤10.0%

Maʻiʻo Peptide:≥80.0%

Hoʻopili a me ka hoʻouna ʻana: Haʻahaʻa haʻahaʻa, hoʻopaʻa haʻahaʻa, pololei i ka mg e like me ka makemake.

Pehea e kauoha ai?

1. Contact us directly by phone or email: +86-13735575465, sales1@gotopbio.com.

2. E kauoha ma ka pūnaewele.E ʻoluʻolu e hoʻopiha i ka palapala kauoha ma ka pūnaewele.

3. Hāʻawi i ka inoa peptide, CAS No. a i ʻole ke kaʻina, ka maʻemaʻe a me ka hoʻololi inā makemake ʻia, ka nui, a me nā mea ʻē aʻe e hāʻawi mākou i kahi ʻōlelo i loko o 2 mau hola.

4. E kauoha i ka conformation e ka aelike kuai i kakau pono ia a me ka NDA (non disclosure agreement) a i 'ole ka aelike huna.

5. E hoʻomau mau mākou i ka holomua o ke kauoha i ka manawa.

6. Hāʻawi ʻia ka peptide e DHL, Fedex a i ʻole nā ​​​​mea ʻē aʻe, a me HPLC, MS, COA me ka ukana.

7. E hahai ʻia ke kulekele hoʻihoʻi inā he ʻokoʻa o kā mākou maikaʻi a lawelawe paha.

8. ʻO ka lawelawe ma hope o ke kūʻai aku: Inā he nīnau kā mākou mea kūʻai e pili ana i kā mākou peptide i ka wā o ka hoʻokolohua, e ʻoluʻolu e kelepona iā mākou a e pane mākou iā ia i ka manawa pōkole.

Hoʻohana wale ʻia nā huahana a pau o ka hui no ka noiʻi ʻepekema, ʻo ia's pāpā ʻia e hoʻohana pololei ʻia e kekahi kanaka ma ke kino kanaka.

FAQ：

Ua hōʻemi ʻia nā peptides i loaʻa iā Cys ma mua o ka hoʻouna ʻana?

Inā ʻaʻole ʻike ʻia ka peptide i hoʻokahe ʻia, ʻaʻole mākou e hōʻemi i ka Cys.Loaʻa nā polypeptides a pau mai nā huahana crude i hoʻomaʻemaʻe ʻia a lyophilized ma lalo o nā kūlana pH2, ka mea ma kahi liʻiliʻi e pale i ka oxidation o Cys.Hoʻomaʻemaʻe ʻia nā peptides i loaʻa iā Cys ma ka pH2 ke ʻole he kumu kikoʻī e hoʻomaʻemaʻe ai i ka pH6.8.Inā hana ʻia ka hoʻomaʻemaʻe ʻana ma ka pH6.8, pono e mālama koke ʻia ka huahana i hoʻomaʻemaʻe ʻia me ka waikawa i mea e pale ai i ka oxidation.Ma ka ʻanuʻu hoʻomalu maikaʻi hope loa, no nā peptides i loaʻa iā Cys, inā ʻike ʻia ka nui o ke kaumaha molekala (2P+H) ma ka palapala ʻāina MS, hōʻike ia ua hana ʻia kahi dimer.Inā ʻaʻohe pilikia me MS a me HPLC, e lyophilize pololei mākou a hoʻouna i nā waiwai me ka ʻole o ka hana hou ʻana.Pono e hoʻomaopopo ʻia ʻo nā peptides e loaʻa ana iā Cys e hoʻoheheʻe lohi i ka manawa, a ʻo ke kiʻekiʻe o ka oxidation e pili ana i ke kaʻina peptide a me nā kūlana mālama.

Pehea ʻoe e hoʻoholo ai inā pili ʻia kahi peptide?

Hoʻohana mākou i ka pane ʻo Ellman e hoʻāʻo ai inā ua pau ke apo.Inā maika'i ka ho'ā'o 'Ellman (melemele), 'a'ole i piha ka pane apo.Inā maikaʻi ʻole nā ​​hopena hoʻāʻo (ʻaʻole melemele), ua pau ka pane apo.ʻAʻole mākou e hāʻawi i ka hōʻike loiloi o ka ʻike cyclization no kā mākou mea kūʻai.ʻO ka mea maʻamau, aia ka wehewehe ʻana i nā hopena hoʻāʻo a Ellman ma ka hōʻike QC.

Pono wau i kahi peptide cyclic, aia kahi tryptophan, e hoʻoheheʻe ʻia?

ʻO ka oxidation o tryptophan he mea maʻamau i ka peptide oxidation, a ʻo ka peptides ka mea maʻamau ma mua o ka hoʻomaʻemaʻe ʻana.Inā hiki mai ka ho'ohāhā o tryptophan, e ho'ololi ka manawa pa'a o ka peptide ma ke kolamu HPLC, a hiki ke ho'opau 'ia ka oxidation e ka ho'oma'ema'e.Eia kekahi, hiki ke ʻike ʻia nā peptides oxidized e MS.

Pono anei e kau i kahi āpau ma waena o ka peptide a me ka wai?

Inā ʻoe e hoʻopili i kahi mole nui (e like me ka dye) i ka peptide, ʻoi aku ka maikaʻi o ka waiho ʻana i kahi ākea ma waena o ka peptide a me ka ligand e hōʻemi i ka hoʻopilikia ʻana i ka mea loaʻa ma o ka pelu ʻana o ka peptide ponoʻī a i ʻole ka pelu ʻana o ka peptide. kona conjugate.ʻAʻole makemake nā poʻe ʻē aʻe i nā wā āpau.No ka laʻana, i ka pelu ʻia ʻana o nā protein, hiki ke hoʻoholo i ka mamao o ke kaʻawale ʻana o ka hoʻopili ʻana o kahi waikawa amino ma o ka hoʻopili ʻana i kahi dye fluorescent i kahi kahua.

Inā makemake ʻoe e hana i kahi hoʻololi biotin ma ka pahu N, pono ʻoe e kau i kahi āpau ma waena o ka biotin a me ke kaʻina peptide?

ʻO ke kaʻina hana lepili biotin maʻamau i hoʻohana ʻia e kā mākou hui e hoʻopili i kahi Ahx i ke kaulahao peptide, a ukali ʻia e biotin.ʻO Ahx kahi pūhui 6-carbon e hana i ka pale ma waena o ka peptide a me ka biotin.

Hiki iā ʻoe ke hāʻawi i kekahi ʻōlelo aʻo e pili ana i ka hoʻolālā ʻana o nā peptides phosphorylated?

I ka piʻi ʻana o ka lōʻihi, e emi mālie ana ka hana hoʻopaʻa ʻana mai ka waikawa amino phosphorylated ma luna.ʻO ke kuhikuhi synthesis mai ka C terminal a i ka N terminal.Manaʻo ʻia nā koena ma hope o ka phosphorylated amino acid ʻaʻole i ʻoi aku ma mua o 10, ʻo ia hoʻi, ʻo ka nui o nā koena amino acid ma mua o ka phosphorylated amino acid mai ka N terminal a i ka C terminal ʻaʻole pono ma mua o 10.

No ke aha ka n-terminal acetylation a me C-terminal amidation?

Ke pale nei kēia mau hoʻololi i ka peptide mai ka hoʻohaʻahaʻa ʻia a ʻae i ka peptide e hoʻohālike i kona kūlana mua o nā alpha amino a me nā hui carboxyl i loko o ka protein makua.