Ka nānā ʻana i nā ʻenehana synthesis like peptide

ʻenehana synthesis like me ka peptide

Ke ulu wikiwiki nei ka noiʻi a me ka hoʻomohala ʻana o nā lāʻau peptide i ka lāʻau lapaʻau.Eia nō naʻe, ua kaupalena ʻia ka hoʻomohala ʻana o nā lāʻau peptide e ko lākou mau ʻano ponoʻī.No ka laʻana, ma muli o ka naʻau kūikawā i ka hydrolysis enzymatic, ua hoʻemi ʻia ka paʻa, a ʻo ka ʻokoʻa o ka conformation steric ka hopena i ka haʻahaʻa haʻahaʻa kikoʻī, haʻahaʻa hydrophobicity, a me ka nele o kahi ʻōnaehana lawe kikoʻī.I mea e lanakila ai i kēia mau peptide, ua manaʻo ʻia nā hopena he nui a me ka hoʻohana kūleʻa ʻana o kahi ʻano peptide kekahi o lākou.

ʻAno peptide (inoa Pelekania: Peptoid) a i ʻole Poly – N – ma kahi o glycine (inoa Pelekania: Poly real – N – substitutedglycine), he hui peptide quasi o ka peptide ma ke kaulahao nui.Hoʻololi ke kaula kalapona ʻaoʻao alpha i ke kaulahao nui naikokene ma mua o ke kaulahao ʻaoʻao.I loko o ka polypeptide mua, ʻo ka hui R o ke kaulahao ʻaoʻao amino acid he 20 mau amino acids like ʻole, akā ʻoi aku ka nui o nā koho o ka hui R i ka peptoid.I ka peptide, peptide ma ke kaulahao nui o nā waikawa amino i loko o ka alpha carbon nitrogen ma kahi o ka hoʻololi ʻana i ke kaulahao ʻaoʻao i ke kaulahao nui.Pono e ʻōlelo ʻia ʻaʻole hana nā peptides i nā hale i kauoha ʻia e like me nā hale kiʻekiʻe ma nā peptides a me nā protein ma muli o ka nele o ka hydrogen ma ka naikokene iwi kuamoʻo.ʻO ka pahuhopu mua ʻo Peptide ka hoʻomohala ʻana i ka peptide kūpaʻa a me ka protease o nā lāʻau mole liʻiliʻi.

Ka nānā ʻana i nā ʻenehana synthesis like peptide

Ua hoʻokomo ʻia ke ʻano o ka synthesis peptide

ʻO ke ʻano hana synthesis e like me ka peptide kaulana loa ʻo ia ke ʻano synthesis subsingle i hana ʻia e RonZuckermann, ua māhele ʻia kēlā me kēia i ʻelua mau ʻanuʻu: acylation a me ka neʻe.I ka acylation, ʻo ka hana mua ka hoʻāla ʻana i ka waikawa haloacetic e hana me nā amine i koe ma ka hopena o ka pae mua, ʻo ka diisopropyl carbonized diimine maʻamau.Ua ho'ā 'ia ka bromoaceticacid e ka diisopropylcarbodiimide."Ma nā hopena hoʻololi (bimolecular nucleophilic substitution reactions), he amine, maʻamau maʻamau, hoʻouka i ka halogen ʻē aʻe e hana i kahi glycine i pani ʻia e N."Ke hoʻohana nei ke ala synthetic subunitary i nā amine mua i loaʻa e hana i nā peptides, a laila e hiki ai i ka synthesis kemika o nā peptides.

ʻO ka hoʻonui paʻa i ka papa peptides synthesis ka ʻike waiwai, hiki iā ia ke hāʻawi iā ʻoe i nā ʻano ʻano lawelawe peptide synthesis.

Ka nānā ʻana i nā ʻenehana synthesis like peptide

ʻO ka maikaʻi o ia peptide

ʻOi aku ka paʻa: ʻoi aku ka paʻa o nā peptoids i loko o vivo ma mua o nā peptides.

ʻOi aku ka koho ʻana: Ua kūpono nā peptoids no nā haʻawina ʻike lāʻau i hui ʻia no ka mea hiki ke loaʻa nā ʻano nui o nā poloka hale polypeptide like ʻole ma ka hoʻololi ʻana i ka hui amino iwi kuamoʻo.

ʻOi aku ka maikaʻi: ʻO ka nui o nā hale peptoid hiki ke hoʻolilo i ka peptoid i koho maikaʻi no ke kaʻina scanning e ʻimi koke i nā hale kikoʻī e pili ana i nā protein.

ʻOi aku ka nui o ka mākeke: ʻo nā hiʻohiʻona o ke ʻano o ka peptide e lilo ia i ʻano o ka hoʻomohala ʻana i ka lāʻau.