Hoʻolālā hoʻolālā a me ka hopena o ke kaulahao peptide polypeptide

I. Hōʻuluʻulu manaʻo
ʻO nā peptide nā macromolecules kūikawā e like me ko lākou mau kaʻina hana ʻokoʻa i kā lākou mau hiʻohiʻona kemika a me ke kino.ʻO kekahi mau peptides he paʻakikī ke synthesize, aʻo nā mea'ē aʻe he maʻalahi ke synthesize akā paʻakikī ke hoʻomaʻemaʻe.ʻO ka pilikia kūpono, ʻo ka hapa nui o nā peptides e hiki ke hoʻoheheʻe ʻia i loko o nā wai wai, no laila, i kā mākou hoʻomaʻemaʻe ʻana, pono e hoʻoheheʻe ʻia ka ʻāpana like o ka peptide hydrophobic i nā solvents non-aqueous. o nā kaʻina hana hoʻokolohua biological, no laila ua pāpā loa ʻia nā ʻenehana mai ka hoʻohana ʻana i ka peptide no kā lākou hana ponoʻī, no laila he mau ʻano o ka hoʻolālā ʻana o nā peptides no nā mea noiʻi.

Hoʻolālā hoʻolālā a me ka hopena o ke kaulahao peptide polypeptide
ʻO ka lua, ke koho pololei o nā peptides paʻakikī synthetic
1. Ka lōʻihi holoʻokoʻa o nā kaʻina i hoʻoponopono ʻia
ʻOi aku ka maʻalahi o ka loaʻa ʻana o nā peptide ma lalo o 15 mau koena no ka mea e piʻi ana ka nui o ka peptide a e emi ana ka maʻemaʻe o ka huahana crude.Ke piʻi nei ka lōʻihi o ke kaulahao peptide ma mua o 20 mau koena, ʻo ka nui o ka huahana ke kumu nui.I nā hoʻokolohua he nui, maʻalahi ka loaʻa ʻana o nā hopena i manaʻo ʻole ʻia ma ka hoʻohaʻahaʻa ʻana i ke koena helu ma lalo o 20.
2. E ho'ēmi i ka helu o nā koena hydrophobic
ʻO nā peptide me ka nui o nā koena hydrophobic, ʻoi aku ka nui ma ka ʻāina 7-12 koena mai ka C-terminus, maʻamau ke kumu i nā pilikia synthetic.ʻIke ʻia kēia ma ke ʻano he hui kūpono ʻole ma muli o ka loaʻa ʻana o kahi pepa B-fold i ka synthesis."Ma ia mau hihia, pono paha e hoʻololi i ʻoi aku ma mua o ʻelua mau koena maikaʻi a maikaʻi ʻole, a i ʻole e hoʻokomo iā Gly a i ʻole Pro i loko o ka peptide e wehe i ka haku mele peptide."
3. Hoʻohaʻahaʻa i nā koena "paʻakikī".
"He nui nā koena Cys, Met, Arg, a me Try i ʻike ʻole ʻia."E hoʻohana pinepine ʻia ʻo Ser ma ke ʻano he koho nonoxidative iā Cys.
Hoʻolālā hoʻolālā a me ka hopena o ke kaulahao peptide polypeptide

ʻO ke kolu, e hoʻomaikaʻi i ke koho kūpono o ka mea hoʻoheheʻe i ka wai
1. Hoʻoponopono i ka palena N a i ʻole C
E pili ana i nā peptides acidic (ʻo ia hoʻi, hoʻopiʻi maikaʻi ʻia i ka pH 7), acetylation (N-terminus acetylation, C terminal e mālama mau ana i kahi pūʻulu carboxyl manuahi) e hoʻonui i ka uku ʻino.Eia nō naʻe, no nā peptides maʻamau (ʻo ia hoʻi, hoʻopiʻi maikaʻi ʻia ma ka pH 7), ʻo ka amination (pūʻulu amino manuahi ma ka N-terminus a me ka amination ma ka C-terminus) ua ʻōlelo nui ʻia e hoʻonui i ka uku maikaʻi.

2. Hoʻopōkole nui a hoʻolōʻihi paha i ke kaʻina

ʻO kekahi o nā kaʻina i loaʻa ka helu nui o nā waikawa amino hydrophobic, e like me Trp, Phe, Val, Ile, Leu, Met, Tyr a me Ala, a pēlā aku.Hiki paha ke hoʻolōʻihi i ke kaʻina no ka hoʻonui hou ʻana i nā pou maikaʻi a maikaʻi ʻole o ka peptide.ʻO ka lua o ka koho e hoʻohaʻahaʻa i ka nui o ke kaulahao peptide e hoʻonui i nā pou maikaʻi a maikaʻi ʻole ma o ka hoʻohaʻahaʻa ʻana i nā koena hydrophobic.ʻOi aku ka ikaika o nā ʻaoʻao maikaʻi a maikaʻi ʻole o ke kaulahao peptide, ʻoi aku ka nui o ka hopena me ka wai.
3. E hoʻokomo i kahi koena hiki ke hoʻoheheʻe ʻia i ka wai
No kekahi mau kaulahao peptide, hiki i ka hui pū ʻana o kekahi mau waikawa amino maikaʻi a maikaʻi ʻole ke hoʻomaikaʻi i ka solubility wai.Manaʻo kā mākou hui i ka N-terminus a i ʻole C-terminus o nā peptides acidic e hui pū me Glu-Glu.Hāʻawi ʻia ka N a C paha o ka peptide kumu a laila ʻo Lys-Lys.Inā ʻaʻole hiki ke kau ʻia ka hui i hoʻopiʻi ʻia, hiki ke hoʻokomo pū ʻia ʻo Ser-Gly-Ser ma ka palena N a i ʻole C.Eia naʻe, ʻaʻole hana kēia ala ke hiki ʻole ke hoʻololi i nā ʻaoʻao o ke kaulahao peptide.