Wahi a ke ala hoʻohui o ka amino acid a me ke kō, hiki ke hoʻokaʻawale ʻia ka peptide kō i ʻehā mau ʻāpana: O glycosylation, C a N glycosylation, dew saccharification a me GPI (glycophosphatidlyinositol) pili.
1. N-glycosylation glycopeptides ua haku ia me ka N-acetamide glucose ma ka hopena hoemi o ke kaulahao glycan (Glc-Nac) i hoopiliia i ka N atom ma ka hui amide o ke kaulahao aoao o kekahi Asn i ke kaulahao peptide, a me ka Asn. hiki ke hoʻopili i ke kaulahao glycan pono e loaʻa i ka AsN-X-Ser /Thr (X! =P) ma ke kumu i hana ʻia e nā koena.ʻO N-acetylglucosamine ke kō.
N-glycosylation hoʻololi structural glycopeptide
2. ʻOi aku ka maʻalahi o ke ʻano o ka O-glycosylation ma mua o ka N-glycosylation.ʻOi aku ka pōkole o kēia glycopeptide ma mua o ka glycan, akā ʻoi aku ka nui o nā ʻano ma mua o ka N-glycosylation.Hiki ke hoʻopili ʻia ʻo Ser a me Thr ma ke kaulahao peptide.Eia kekahi, aia nā glycopeptides i kāhiko ʻia me ka tyrosine, hydroxyl, a me ka hydroxyproline glycosylation.ʻO ke kūlana loulou ka hydroxyl oxygen atom ma ke kaulahao ʻaoʻao o ke koena.ʻO nā kō i hoʻopili ʻia he galactose a i ʻole N-acetylgalactosamine (Gal&GalNAc) a i ʻole glucose/glucosamine (Glc/GlcNAc), mannose/mannosamine (Man/ManNAc), etc.
Hoʻololi ka O-glycosylation i ke ʻano
3. Glycopeptide O-GlcNAC glycosylation ((N-acetylcysteine (NAC)) (glcnAcN-acetylglucosamine/acetylglucosamine)
Hoʻokahi N-acetylglucosamine (GlcNAc) glycosylation e hoʻopili i nā protein O-GlcNAc i ka hydroxyl oxygen atom o ka serine a i ʻole threonine koena o kahi protein.ʻO ka glycosylation O-GlcNA he mea hoʻonani monosaccharide O-GlcNAc me ka hoʻonui ʻole glycan;E like me ka phosphorylation peptide, ʻo O-GlcNAc glycosylation o glycopeptides kahi hana hoʻonaninani protein ikaika.Hiki ke hoʻoulu ʻia ka hoʻonaninani O-GlcNAc maʻamau i nā maʻi like ʻole e like me ka maʻi diabetes, nā maʻi cardiovascular, nā ʻōpū, ka maʻi Alzheimer a pēlā aku.
Nā wahi Glycosylation o nā glycopeptides
Hoʻopili ʻia nā ʻano kumu o ka polypeptide a me nā kaulahao kō i nā kaulahao protein e nā kaula covalent, a ʻo nā pūnaewele e pili ana i nā kaulahao kō i kapa ʻia nā pae glycosylation.No ka mea ʻaʻohe kumu hoʻohālike e hahai i ka biosynthesis o nā kaulahao kōpaʻa glycopeptide, e hoʻopili ʻia nā kaulahao kō like ʻole i ka pae glycosylation hoʻokahi, e alakaʻi ana i ka microscopic inhomogeneity.
ʻO Glycosylation o nā glycopeptides
1. Ka hopena o ka glycopeptide glycosylation i ka therapy-efficacy o nā protein therapeutic
I ka hihia o nā protein therapy-therapeutic, pili pū ka glycosylation i ka hapalua o ke ola a me ka huli ʻana o nā lāʻau protein i vivo
2. Hoʻoheheʻe glycopeptide glycosylation a me nā protein
Ua hōʻike ʻia nā haʻawina e hiki i nā kaulahao kō ma ka ʻili o nā protein ke hoʻomaikaʻi i ka molecular solubility o nā protein
3. Glycopeptide glycosylation a me ka protein immunogenicity
Ma kekahi ʻaoʻao, hiki i nā kaulahao kō ma ka ʻili o nā protein ke hoʻoulu i nā pane kūlohelohe kūikawā.Ma ka ʻaoʻao ʻē aʻe, hiki i nā kaulahao kō ke uhi i kekahi mau wahi ma ka ʻili protein a hoʻemi i kona immunogenicity
4. Glycopeptide glycosylation e hoʻonui i ka paʻa o ka protein
Hiki i ka Glycosylation ke hoʻonui i ka paʻa o nā protein i nā ʻano denaturation like ʻole (e like me nā denaturants, wela, a me nā mea ʻē aʻe) a pale i ka hōʻuluʻulu ʻana o nā protein.I ka manawa like, hiki i nā kaulahao kō ma ka ʻili o nā protein ke uhi i kekahi mau wahi hoʻohaʻahaʻa proteolytic o nā molekala protein, a laila e hoʻonui ai i ke kūʻē ʻana o nā protein i nā proteinases.
5. ʻO Glycopeptide glycosylation e pili ana i ka hana olaola o nā molekole protein
Hiki i ka hoʻololi ʻana i ka glycosylation protein ke hiki i nā molekole protein ke hana i nā hana olaola hou
Ka manawa hoʻouna: ʻAukake-03-2023