ʻO Integrin, a i ʻole integrin, he heterodimer transmembrane glycoprotein receptor e hoʻopili ana i ka hoʻopili ʻana o nā cell cell adhesion a me ka hōʻailona.Ua haku ʻiaα aβ nā ʻāpana liʻiliʻi.Hoʻopili ia i ka optimization o nā hana kelepona like ʻole me ka neʻe ʻana o ka cell, ka infiltration cell, cell and intercellular signaling, cell adhesion, a me nā kaʻina angiogenesis.Hoʻohuiαvβ3 i kēia manawa ua ʻike nui ʻia.ʻO ke ʻano o ka integrinαvβ3 pili loa i ka neʻe ʻana o ka maʻi tumora, ka angiogenesis, ka mumū a me ka osteoporosis.Hōʻike nui ʻia ʻo Integrin i nā ʻiʻo tumora āpau a me nā membrane cell endothelial o neovascularization.ʻO ka hiʻohiʻona o ka integrin pili pili i ka neʻe ʻana o ka tumora a me ka angiogenesis.I nā makahiki i hala iho nei, ua hōʻike ʻia nā noiʻi ʻepekema aia he 11 integrins e hiki ke hoʻopaʻa pono i ka RGD peptide, ʻo ia nā peptides antagonistic no nā mea loaʻa integrin.
Hoʻokaʻawale ʻia ka RGD peptide i loko o ka linear RGD peptide a me ka RGD cyclic peptide.Hoʻohālikelike ʻia me ka linear RGD peptide, RGD cyclic peptide i ʻoi aku ka ikaika o ka hoʻopili ʻana a me ka kikoʻī kikoʻī.Eia nā ʻano maʻamau a me nā ʻano synthesis o RGD cyclic peptide.
ʻO nā ʻano maʻamau o RGD cyclic peptides:
1. Cyclic peptides i loaʻa nā kaʻina RGD i hana ʻia e nā paʻa disulfide
2. Cyclic peptides i loaʻa nā kaʻina RGD i hana ʻia e nā kaula amide
Hoʻohui ʻia o RGD cyclic peptide:
ʻO ka hiʻohiʻona pono e pili ana i kahi kaʻina hana synthesis RGD cyclic peptide ma ke kahua o ka ʻenehana synthesis polypeptide paʻa.ʻO ke ala hou ke koho ʻana i ka resin 2-chloro-triphenylmethyl chloride ma ke ʻano he mea lawe mua, e hoʻopili mua i ka hui carboxyl kaulahao mua me kahi hui pale kūikawā o D aspartic acid amino acid, a laila hoʻopili i ka peptide linear o RGD sequence peptide i ka resin. , a me ka waikawa amino hope loa e wehe i ka hui pale FMOC me ka piperidine.Hoʻohui ʻia ka catalyst e wehe i ka hui pale carboxyl kaulahao ʻaoʻao o ka D aspartic acid mua pololei mai ka resin, a ukali ʻia e ka hoʻohui ʻana o ka piperidine e wehe i ka pūʻulu pale amino FMOC o ka waikawa amino hope, a ukali ʻia e ka hoʻohui ʻana o ka mea paʻa. e hoʻomaʻemaʻe a hoʻopaʻa i ka hui carboxyl a me ka hui amino i hōʻike ʻia mai ke poʻo a me ka hopena o ka peptide laina pololei mai ka resin ma ke ʻano o ka amide bond e hana i ka peptide cyclic.ʻO ka hope, ʻoki pololei ʻia ka peptide cyclic mai ka resin me ka hopena ʻoki.
Ka manawa hoʻouna: Apr-24-2023