Hoʻololi i ka methylation

ʻO nā peptides i hoʻololi ʻia me ka methylation, ʻike ʻia hoʻi ʻo methylation-recognized peptides, he protein post-translational decors (PTMs) a ke pāʻani nei i kahi kuleana hoʻoponopono koʻikoʻi i nā hana ola āpau i loko o nā cell.Hoʻopili ʻia nā protein e ka methyltransferase e hoʻololi i nā pūʻulu hydroxyl i nā koena amino acid kikoʻī no ka paʻa covalent.ʻO ka methylation kahi kaʻina hana hoʻololi i hoʻololi ʻia e nā demethylases.

Ua hōʻike nā haʻawina ʻo ka methylated/demethylated amino acid maʻamau ka lysine (Lys) a me ka arginine (Arg).Ua ʻike nā haʻawina he ʻano hana olaola like ʻole ka histone lysine methylation e like me ka mālama ʻana a me ka hoʻokaʻawale ʻana i nā cell cell, X chromosome inactivation, transcription regulation a me ka pane ʻino DNA.", pili pinepine i ka condensation chromatin a hoʻopaʻa i ka hōʻike gene."He kuleana koʻikoʻi ka Histone arginine methylation i ka hoʻoponopono ʻana i ka transcription gene a hiki ke hoʻopilikia i nā kaʻina hana physiological i loko o nā cell, me ka hoʻoponopono DNA, ka hoʻololi ʻana i ka hōʻailona, ​​​​ka ulu ʻana o ka cell, a me ka carcinogenesis.No laila, ua hoʻomohala kūikawā ʻo Guopeptide Biology i ka ʻenehana o methyl decorative peptides, i hoʻololi ʻia e nā ʻepekema ma hope o ka unuhi ʻana o ka protein (PTMS) e kōkua i ka noiʻi.

Hoʻololi methylation (Me1, Me2, Me3)

Fmoc-Lys(Me,Boc)-OH, Fmoc-Lys(Me2)-OH, Fmoc-Lys(Me3)-OH.HCL, Fmoc-Arg(Me,Pbf)-OH, Fmoc-Arg(Me) Ua hoʻohana ʻia ʻo 2-OH.HCl(asymmetrical), F moc-Arg(me)2-OH.HCl(symmetrical) a ua hana ʻia nā mea maka ʻē aʻe e ka FMOC solid-phase synthesis process e loaʻa ai nā Lys a me Arg methylated peptides, a me nā huahana. ua hoʻomaʻemaʻe ʻia e HPLC.Hāʻawi ʻia nā spectra mass pili, HPLC chromatograms a me COA no ka huahana i hoʻopau ʻia.