Loaʻa pinepine nā antigens protein recombinant i nā epitopes like ʻole, ʻo kekahi o ia mau epitopes sequence a ʻo kekahi he epitopes structural.ʻO nā antibodies polyclonal i loaʻa ma ka hoʻomaʻamaʻa ʻana i nā holoholona me nā antigens denatured he hui pū ʻana o nā antibodies kikoʻī i kēlā me kēia epitopes a hiki ke hoʻohana ʻia ma ka laulā e ʻike i nā hale kūlohelohe a i ʻole nā protein target denatured.ʻO kahi pōmaikaʻi ʻaoʻao o ka hoʻohana ʻana i nā protein denatured ma ke ʻano he immunogens ʻo ia ka mea i ʻoi aku ka immunogenic o nā protein denatured a hiki ke hoʻoulu i ka pane kūlohelohe ikaika i nā holoholona.
ʻO ka ʻōnaehana hōʻike o Escherichia coli e koho pinepine ʻia no nā kumu antigenic no ka mea ʻo ia ka ʻōnaehana kumu kūʻai nui loa i ka manawa a me ke kālā.I mea e hoʻomaikaʻi ai i ka hiki ke hōʻike i ka protein target a me ka maʻalahi o ka hoʻomaʻemaʻe ʻana, i kekahi manawa he ʻāpana liʻiliʻi wale nō o ka protein target, e like me kahi kikowaena kikoʻī.
ʻO ke ʻano ʻekolu-dimensional o nā protein
ʻO ke kahua kikoʻī o kahi protein
Inā ʻo ke kumu o ka hoʻomākaukau ʻana i ka antibody no ka ʻike wb wale nō, ʻoi aku ka maikaʻi a me ka wikiwiki e hoʻohana i ka peptide liʻiliʻi synthetic e like me antigen, akā aia kahi pilikia o ka immunogenicity nāwaliwali a i ʻole ka regenicity ma muli o ke koho kūpono ʻole o ka māhele peptide.Ma muli o ka hoʻomākaukau ʻana o ka antibody i kahi manawa lōʻihi, koho pinepine ʻia nā ʻāpana peptide ʻelua a ʻekolu paha e hoʻomākaukau i nā antibodies me ka hoʻohana ʻana i ka polypeptide antigen e hōʻoia i ka holomua o ka hoʻokolohua.
Pono ka maʻemaʻe o ka polypeptide antigen no ka maʻi maʻi ma mua o 80%.ʻOiai hiki i ka maʻemaʻe kiʻekiʻe ke kiʻi i nā antibodies me ka kikoʻī ʻoi aku ka maikaʻi, ma ka hoʻomaʻamaʻa, hoʻopuka mau nā holoholona i ka nui o nā antibodies non-specific, no laila e hūnā i nā pono o ka maʻemaʻe antigen.
Eia kekahi, ʻo ka hoʻomākaukau ʻana o nā antibodies mai nā peptides liʻiliʻi e pono e hoʻopili ʻia i ka antigen carrier kūpono e hoʻonui ai i kona immunogenicity.ʻElua mau mea lawe antigenic maʻamau ʻo KLH a me BSA.
Ka manawa hoʻouna: Mar-23-2023