Hoʻohālikelike ʻia nā lāʻau peptide ma ke ʻano he polymers i haku ʻia me nā kaula amide me ka liʻiliʻi o 40 mau koena amino acid.Ma muli o ka hana hoʻokipa kiʻekiʻe a me ke koho ʻana o nā lāʻau peptide me ka haʻahaʻa haʻahaʻa o nā hopena ʻaoʻao, ua nui ka hoihoi i nā peptides mai ka ʻoihana lāʻau.I loko o kēia manawa, ua nui nā lāʻau lapaʻau hōkū, i hoʻopaʻa nui ʻia i ka ʻoihana maʻi metabolic, e like me GLP-1 analogue somalutide, gastric inhibitory peptide (GIP) glucagon-like peptide-1 (GLP-1) tesiparatide a me nā mea ʻelua. - nā agonists hoʻokipa.Eia kekahi, me ka piʻi ʻana o nā lāʻau lapaʻau PDC a me RDC.I kēia manawa, ʻo ka hoʻomākaukau ʻana o nā lāʻau lapaʻau polypeptide ka nui o ka synthesis kemika a me ka fermentation biological.Hoʻohana nui ʻia ka biofermentation e hana i nā peptides lōʻihi.ʻO nā mea maikaʻi he mau kumukūʻai haʻahaʻa haʻahaʻa, akā ʻo ka hiki ʻole ke hoʻokomo i nā waikawa amino kūlohelohe i loko o ke kaʻina peptide a me ka hiki ʻole ke hana i nā mea hoʻonaninani like ʻole ma ke kaulahao peptide.No laila, ua kaupalena nui ʻia kāna noi.Hoʻokomo ʻia nā ʻano hana hoʻoheheʻe kemika i ka synthesis pae paʻa a me ka synthesis ʻāpana wai.ʻOi aku ka maikaʻi o ka synthesis solid-phase ma mua o ka synthesis-phase synthesis: hiki ke hoʻohana ʻia kahi ʻoi aku o nā mea no ka pane ʻana e hōʻoia i ka hoʻopili piha ʻana.Hiki ke hoʻoneʻe ʻia nā ʻakika amino keu, nā mea hoʻohaʻahaʻa, a me nā huahana e nā hana hoʻomaʻemaʻe maʻalahi, e pale aku i ka paʻakikī post-processing a me ka hoʻomaʻemaʻe ʻana a me ka hoʻomaikaʻi ʻana i ka hana hana, no laila ua hoʻohana nui ʻia ke ʻano hana synthesis solid-phase."ʻO nā kumu waiwai no ka synthesis o nā peptides nā mea hoʻomaka, reagents, a me nā solvents."ʻO ko lākou maikaʻi, ʻoi aku ka maikaʻi o ka mea mua, hiki ke loaʻa i kahi hopena ʻē aʻe i ka maikaʻi o ka API.ʻO ka mea hoʻomaka e pili nui ana i nā derivatives amino acid i hoʻopaʻa ʻia no ka peptide chain modified fatty acids, polyethylene glycol, etc. Ma ke ʻano he mau ʻāpana kūkulu koʻikoʻi, ua helu ʻia lākou e like me nā mea i loko o ka hoʻolālā API, e pili pono ana i ka maikaʻi o ka API.No laila, pono mākou e nānā i ka mana o ka mea hoʻomaka.
I. E hoʻopololei i ke koho mea mua
Manaʻo maopopo ʻo ICHQ11 inā hoʻohana ʻia kahi huahana kemika i kūʻai ʻia ma ka mākeke ma ke ʻano he kumu mua, ʻaʻole pono ka mea noi e kūkākūkā i kona kūpono.ʻAʻole hiki ke hoʻohana ʻia nā huahana kemika i kūʻai ʻia ma ka mākeke ʻaʻole wale i mea hoʻomaka no nā lāʻau lapaʻau, akā hiki ke kūʻai ʻia aku ma nā mākeke non-pharmaceutical.ʻAʻole pili nā pūhui i hana ʻia a synthesized i nā huahana kemika i kūʻai ʻia ma ka mākeke.ʻOiai ʻaʻohe mākeke lāʻau lapaʻau ʻole no ka pale ʻana i nā waikawa amino e hoʻokō i ka wehewehe ICHQ11 o nā kemika i kūʻai ʻia ma ka mākeke, ua paʻa, ʻokoʻa kemika a maopopo ke ʻano, maʻalahi e hoʻokaʻawale a hoʻomaʻemaʻe, a hiki ke ʻike ʻia a hoʻāʻo ʻia e nā ʻano analytical maʻamau. .Loaʻa iā lākou nā waiwai kemika paʻa a maʻalahi e mālama, lawe, a synthesize
Ii.Ka hoomalu ana i na mea pili i ka mea hoomaka
Hoʻokomo ʻia nā amino acids palekana i ʻōlelo ʻia i loko o ka hoʻolālā API ma ke ʻano he ʻāpana koʻikoʻi, pili pono i ka maikaʻi o ka API.No laila, pono mākou e hoʻomalu pono i ka maʻiʻo haumia i loko o ka mea mua, e hoʻomaopopo i ka hoʻololi a me ka weheʻana i kēia mau mea haumia i loko o ke kaʻina hana i hoʻokumuʻia, a ma hope e wehewehe i ka pilina ma waena o lākou a me nā haumia i loko o ka API.
Ka hoʻomaopopo ʻana i nā mea hoʻomaka lāʻau polypeptide
ʻO ke kolu, ke koena solvent i ka mea mua
Ma keʻano laulā, hāʻawi ʻia i ka kikoʻī o ka hoʻokumu ʻana i ka pae paʻa o nā peptides, e hoʻohana ʻia ka nui o ka solvent e hoʻomaʻemaʻe i ka resin peptide ma hope o ka hoʻopau ʻana i kēlā me kēia kaʻina o ka hui ʻana o ka amino acid a me ka wehe ʻana mai ka pale.ʻO nā peptides kino i loaʻa ma ka haki ʻana i ka resin peptide e hana pū ʻia e HPLC a maloʻo maloʻo.No laila, ʻaʻohe pilikia e hāʻawi ʻia ka liʻiliʻi o ka solvent i hoʻopili ʻia i nā waikawa amino pale i ka API hope.Eia nō naʻe, pono e uku nui ʻia i nā koena o ka acetate, butyl acetate, a me nā mea hoʻoheheʻe waiʻona, no ka mea, hiki i kēia mau mea hoʻoheheʻe ke hana i nā hopena ʻaoʻao me nā ʻakika amino ikaika a i ʻole nā kaulahao peptide i ka wā e hui pū ai me nā waikawa amino.No ka laʻana, i ka wā o ka hui ʻana o ka amino acid, e hoʻopili ke koena acetic acid me ka hui amino i hōʻike ʻia ma ke kaulahao peptide, e hopena i ka hopena pani o ke kaulahao peptide;I ka wā o ka hana amino acid, hiki i ke koena mea hoʻoheheʻe waiʻona ke hana me ka pūʻulu carboxyl ikaika, e alakaʻi ana i ka passivation o ka waikawa amino ikaika, e hōʻemi ana i ka like o ka amino acid, a hopena i ka hui pū ʻana o ka amino acid a me ka nele o nā peptide haumia.Mālama ka hui i ka butyl acetate, ka waiʻona, ka methanol, a me ka acetic acid i COA, e lawe ana i kahi amino acid mai Zheng Yuan Biochemical i laʻana.ʻO ka maʻamau no ka butyl acetate he ≤0.5% butyl acetate, i ʻike maoli ʻia ʻo 0.10%.Wahi a ka ICHQ3C, butyl acetate no nā ʻano solvents ʻekolu, hoʻonohonoho i ka maʻamau no 0.5% a i ʻole ka liʻiliʻi e like me nā koi o ICHQ3C, akā no ka noʻonoʻo ʻana i ka butyl acetate amino acetylation hiki ke alakaʻi i ka pilikia, e hana pū me ka butyl acetate e hoʻohālikelike i ka noiʻi. , e hoʻoholo i kahi kūlana kūpono.
Ka manawa hoʻouna: ʻAukake-29-2023