He aha ka hana o ka phosphorylation i nā peptides?

Hoʻopili ka Phosphorylation i nā ʻano āpau o ke ola kelepona, a ʻo ka protein kinases e hoʻopili i nā ʻano āpau o ka hana kamaʻilio intracellular ma o ka hoʻoponopono ʻana i nā ala hōʻailona a me nā kaʻina kelepona.Eia naʻe, ʻo ka phosphorylation aberrant ke kumu o nā maʻi he nui;ʻo ia hoʻi, hiki i ka mutated protein kinases a me ka phosphatases ke hoʻoulu i nā maʻi he nui, a he nui nā toxins kūlohelohe a me nā pathogens i loaʻa ka hopena ma ka hoʻololi ʻana i ke kūlana phosphorylation o nā protein intracellular.

ʻO ka Phosphorylation o serine (Ser), threonine (Thr), a me tyrosine (Tyr) kahi kaʻina hana hoʻololi protein reversible.Hoʻopili lākou i ka hoʻoponopono ʻana i nā hana kelepona he nui, e like me ka hōʻailona hōʻailona, ​​​​ka hui pūmua a me ka hoʻokaʻawale ʻana, ka hoʻoulu ʻana a i ʻole ka pale ʻana i ka hana protein, a me ke ola pū kekahi.Hoʻopiʻi maikaʻi ʻia nā Phosphates (ʻelua mau uku maikaʻi ʻole no ka hui phosphate).No laila, hoʻololi kā lākou hoʻohui i nā waiwai o ka protein, ʻo ia ka mea maʻamau i ka hoʻololi conformational, e alakaʻi ana i kahi hoʻololi i ke ʻano o ka protein.Ke wehe ʻia ka pūʻulu phosphate, e hoʻi ka conformation o ka protein i kona kūlana mua.Inā hōʻike nā protein conformational ʻelua i nā hana like ʻole, hiki i ka phosphorylation ke hana ma ke ʻano he hoʻololi molekala no ka protein e hoʻomalu i kāna hana.

Nui nā hormones e hoʻoponopono i ka hana o nā enzyme kikoʻī ma o ka hoʻonui ʻana i ke kūlana phosphorylation o serine (Ser) a i ʻole threonine (Thr) koena, a hiki ke hoʻoulu ʻia ka tyrosine (Tyr) phosphorylation e nā mea ulu (e like me ka insulin).Hiki ke wehe koke ʻia nā pūʻulu phosphate o kēia mau amino acid.No laila, hana ʻo Ser, Thr, a me Tyr ma ke ʻano he hoʻololi molekala i ka hoʻoponopono ʻana i nā hana kelepona e like me ka hoʻonui ʻana o ka tumora.

ʻO nā peptides synthetic kahi hana maikaʻi loa i ke aʻo ʻana i nā substrates kinase protein a me nā pilina.Eia nō naʻe, aia kekahi mau mea e keʻakeʻa a kaupalena paha i ka adaptability o ka ʻenehana synthesis phosphopeptide, e like me ka hiki ʻole ke hoʻokō i ka automation piha o ka synthesis solid-phase a me ka nele o ka pilina kūpono me nā paepae analytical maʻamau.

Ua lanakila ka ʻenehana peptide synthesis a me ka hoʻololi phosphorylation i kēia mau palena i ka hoʻomaikaʻi ʻana i ka pono synthesis a me ka scalability, a ua kūpono ke kahua no ke aʻo ʻana i nā substrate kinase protein, antigens, binding mole, a me nā mea hoʻopaneʻe.