He mau peptide liʻiliʻi nā peptides e komo ana i ka cell e hiki ke komo maʻalahi i ka membrane cell.ʻO kēia papa o nā molekala, ʻo ia hoʻi nā CPP me nā hana i hoʻopaʻa ʻia, paʻa i ka ʻōlelo hoʻohiki no ka hāʻawi ʻana i ka lāʻau lapaʻau i nā cell target.
No laila, ʻo ka noiʻi ʻana ma luna o ia mea he koʻikoʻi biomedical.I loko o kēia haʻawina, ua aʻo ʻia nā CPP me nā hana transmembrane like ʻole ma ka pae kaʻina, e hoʻāʻo ana e ʻike i nā mea e pili ana i ka hana transmembrane o CPPs, nā ʻokoʻa o ke kaʻina ma waena o nā CPP me nā hana like ʻole a me nā NonCPPs, a hoʻolauna i kahi ala no ka nānā ʻana i nā kaʻina olaola.
Loaʻa nā CPP a me NonCPPs mai ka waihona CPPsite a me nā palapala like ʻole, a me nā peptides transmembrane (HCPPs, MCPPs, LCPPs) me ka hana transmembrane kiʻekiʻe, waena, a haʻahaʻa i unuhi ʻia mai nā kaʻina CPPs e kūkulu i nā pūʻulu ʻikepili.Ma muli o kēia mau ʻikepili, ua hana ʻia nā haʻawina penei:
1, Ua kālailai ʻia ka ʻakika amino a me ka haku mele lua o nā CPP ikaika a me nā NonCPP e ANOVA.Ua ʻike ʻia he mea koʻikoʻi ka pilina electrostatic a me ka hydrophobic o nā waikawa amino i ka hana transmembrane o CPPs, a ʻo ke ʻano helical a me ka coiling random i pili pū i ka hana transmembrane o CPPs.
2. Ua hōʻike ʻia nā waiwai kino a me nā kemika a me nā lōʻihi o nā CPP me nā hana like ʻole ma ka mokulele ʻelua.Uaʻikeʻia e hiki ke hoʻopiliʻia nā CPP a me nā NonCPP me nā hana likeʻole ma lalo o kekahi mau waiwai kūikawā, a ua māheleʻia nā HCPP, MCPP, LCPP a me NonCPP iʻekolu hui, e hōʻike ana i ko lākou mauʻokoʻa;
3. Ma keia pepa, ua hookomo ia ka manao o ke kino a me kemika centroid o ke kaina olaola, a ua manaoia na koena e haku ana i ke kaina ma ke ano he mau kiko liilii, a ua unuhiia ke ka'ina ma ke ano he oihana no ka noii.Ua hoʻohana ʻia kēia ʻano hana i ka nānā ʻana i nā CPP ma ka hoʻolālā ʻana i nā CPP me nā hana like ʻole ma ka mokulele 3D ma ke ʻano PCA, a ua ʻike ʻia ʻo ka hapa nui o nā CPP i hui pū ʻia a hui pū kekahi mau LCPP me NonCPPs.
Loaʻa i kēia haʻawina nā manaʻo no ka hoʻolālā ʻana i nā CPP a me ka hoʻomaopopo ʻana i nā ʻokoʻa o nā kaʻina o nā CPP me nā hana like ʻole.Eia kekahi, hiki ke hoʻohana ʻia ke ʻano loiloi o ka centroid kino a me kemika o nā kaʻina olaola i hoʻokomo ʻia ma kēia pepa no ka nānā ʻana i nā pilikia olaola ʻē aʻe.I ka manawa like, hiki ke hoʻohana ʻia e like me nā ʻāpana hoʻokomo no kekahi mau pilikia hoʻokaʻawale biological a hoʻokani i kahi kuleana i ka ʻike kumu.
Ka manawa hoʻouna: Iune-15-2023