He aha ke ʻano o nā peptides i hana ʻia?ʻIke ʻoe i kēia mau wahi?

Ua hoʻohana nui ʻia ka peptide chain synthesis i nā kula he nui, ʻoi aku ka nui o ka hoʻomohala ʻana i ka lāʻau lapaʻau, ka noiʻi biological a me ka biotechnology.Hiki ke hoʻohui ʻia nā peptide o nā lōʻihi like ʻole a me nā kaʻina ma o ka peptide chain synthesis no ka hoʻomākaukau ʻana i ka lāʻau lapaʻau, lawe lāʻau lapaʻau, kānana protein, noiʻi hana a pēlā aku.No laila he aha ke kuhikuhi o nā peptides synthetic?I kēia lā, hāʻawi ʻo Gutuo Xiaobian iā ʻoe i kahi pane kikoʻī ma lalo nei.

He aha ke ʻano o nā peptides i hana ʻia?Maopopo iā ʻoe kēia mau wahi?

Hiki ke hoʻokō ʻia ka synthesis kaulahao peptide e ka hoʻohui stepwise o nā molekole amino acid i ke kaulahao peptide e kū nei, e hana ana i nā paʻa peptide hou.Ma muli o ka ulu ʻana mai ka hopena N a hiki i ke kaulahao peptide C, no laila ʻo ke kuhikuhi o ka synthesis kekahi mai ka hopena N a i C. ʻO kēia ke kumu no ka C terminal a me ka N terminal amino acids ma waena o ke kaʻina hana peptide bond, nā amino acids o ka hopena carboxyl. (C) a me ka amino (N) i kēia manawa ma ka hopena o ka hopena o ke kaulahao peptide, e hana i kahi paʻa peptide hou.No laila, ke kuhikuhi o ka synthesis mai ka hopena N a i C.

ʻO ka peptide synthesis ka mea nui e hoʻopili ai i nā molekole amino acid ma o ke ʻano kemika e hana i kahi kaʻina o nā peptides.No ka kikoʻī, hoʻopili ka polypeptide chain synthesis i nā molekole amino acid, ma ka huli ʻana, hiki ke hana ʻia e nā mea paʻa kemika e kūkulu i kahi kaʻina o nā peptides.Hiki ke hoʻokō ʻia ka synthesis kaulahao peptide e ka synthesis paʻa-phase a i ʻole synthesis wai-phase.I loko o ka synthesis solid-phase, hoʻopili ʻia nā amino acids mua i ka mea paʻa-phase, a laila hoʻonui ʻia ke kaulahao peptide e ka hoʻohui stepwise o kēlā me kēia amino acid.

Aia ma luna ka mea liʻiliʻi e hoʻolauna i ka hoʻokaʻawale ʻana o ka peptide a me ke kuleana o ka ʻike pili, ka tinto paʻa i ka peptide synthesis, ka ʻōlelo protein, ka hoʻomākaukau ʻana i ka antibody, a me nā mea ʻē aʻe. , hoʻokipa i ka lehulehu o nā mea kūʻai aku e hele mai e kūkākūkā.